The Bendigo-based Loddon Mallee regional office will merge with the Coburg-based Northern Metropolitan region headquarters.
The Community and Public Sector Union has confirmed at least 41 jobs will go from the Bendigo office, with department sources quoting figures close to 100.
The proposed statewide restructure will see nine regions merged into four.
CPSU organiser Nick Church said the job cuts would hit the department, schools and the general community.
“We are hearing numbers of a similar nature,” he said.
“We have at least 41 on a list. When you include that with what we are hearing on the ground, then that number of people, 100, could be true.”
Mr Church said staff could know their fate as early as next week.
“They are merging Loddon Mallee Region with Northern Metropolitan Region. We don’t know where people will end up and who will lose their jobs. We just don’t know.
“The government will confirm nothing until the end of the consultation period, which is September 28.
“We are talking about a significant amount of people.”
Mr Church said positions had already been announced to go from local literacy and numeracy programs, reading recovery, drug education
programs, and refugee support programs.
“The government is claiming it is doing this to be more efficient. We reject all of that,” he said.
“Our concern is of the effect on the Victorian community, especially in the regions, as a result of these cuts.
“It will have a negative effect. Front line people will now be doing back-office roles as well which takes them off the front line.”
The Weekly also understands there is a fear teacher and principal support services, such as mentoring and coaching, would go under the cuts.
A spokesman for Education Minister Martin Dixon was unavailable for comment.